GSTRAP 4B-色谱柱 -耗材-生物在线
上海基星生物科技有限公司
GSTRAP 4B

GSTRAP 4B

商家询价

产品名称： GSTRAP 4B

英文名称： GSTRAP 4B

产品编号： 28-4017-45

产品价格： 0

产品产地： 美国

品牌商标： GE

更新时间： null

使用范围： null

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28-4017-45 GSTRAP 4B, 5 X 1 ML
28-4017-46 GSTRAP 4B, 100 X 1 ML
28-4017-47 GSTRAP 4B, 1 X 5 ML
28-4017-48 GSTRAP 4B, 5 X 5 ML
28-4017-49 GSTRAP 4B, 100 X 5 ML
28-4058-44 HiTrap Capto Adhere 5x1ml
28-4058-46 5x5 ml Hitrap Capto adhere
28-4082-53 HITRAP MABSELECT, 5 X 1 ML
28-4082-55 HITRAP MABSELECT, 1 X 5 ML
28-4082-56 HITRAP MABSELECT, 5 X 5 ML
28-4082-58 HITRAP MABSELECT XTRA, 5X1ML
28-4082-60 HITRAP MABSELECT XTRA, 1X5ML
28-4082-61 HITRAP MABSELECT XTRA, 5X5ML
28-4110-01 HiTrap Butyl HP, 5x1ml
28-4110-05 HiTrap Butyl HP, 5 x 5ml
28-4110-07 HiTrap HIC Selection Kit, 7x1
28-9075-46 StrepTrap HP 5 x 1mL
28-9075-47 StrepTrap HP 1 x 5mL
28-9075-48 StrepTrap HP 5 x 5mL
28-9165-37 HiTrap Capto DEAE, 5X1ML
28-9165-40 HiTrap Capto DEAE, 5X5ML
28-9187-78 MBPTrap 5 x 1mL
28-9187-79 MBPTrap 1 x 5 mL
28-9187-80 MBPTrap 5 x 5mL
28-9343-88 HiTrap Capto IEX Selection Kit
28-9520-85 HiTrap Con A 4B 5 x 1 ml
28-9520-96 HiTrap Con A 4B 5 x 5 ml

Glutathione Sepharose™ 4B Media/GSTrap 4B Columns - Easy Purification

  • Fast and simple purification of GST*-tagged proteins.
  • GSTrap™ 4B 1-ml and 5-ml columns are prepacked with Glutathione Sepharose™ 4B.
  • GSTrap™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTAdesign

Glutathione Sepharose™ 4B Media/GSTrap 4B Columns - Easy Purification

Technical Information


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GSTrap 4B 1-ml and 5-ml columns are prepacked with Glutathione Sepharose 4B for fast purification of GST-tagged proteins and scale-up.

 

GSTrap™ 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.

 

For an introduction to Glutathione S-transferase (GST) Gene Fusion System, see Introduction to Glutathione S-Transferase (GST) Gene Fusion System.

 

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Columns: Capture   GSTrap 4B 1 ml
Polishing   HiLoad 16/60 Superdex 200 pg
Sample: Cell extract from E. coli expressing GST-hippocalcin (Mr 45 000)
after lysis with CelLytic Express
AC binding/wash buffer: 10 mM sodium phosphate, 140 mM, pH 7.4
AC elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
GF buffer: 50 mM phosphate buffer, 150 mM NaCl, pH 7.2
System: ÄKTAprime plus

 

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Click here to view this image in high resolution
1 Low molecular weight markers
2 Start material (diluted 1:20)
3 Eluted pool GSTrap 4B
4 Eluted pool GSTrap 4B (diluted 1:50)
5 Peak 1, HiLoad 16/60 Superdex 200 pg
6 Peak 2, HiLoad 16/60 Superdex 200 pg

 

TECHNICAL SPECIFICATIONS
Ligand  Glutathione 
Ligand coupling method  Epoxy activation 
Dynamic binding capacity*  > 5 mg horse liver GST/ml medium 
Matrix  Agarose, 4% 
Average particle size  90 μm 
pH stability  4-13 
Storage  20% ethanol 
Storage temperature  4°C to 30°C 
HiTrap prepacked columns
Column volume  1 ml and 5 ml 
Column dimensions  0.7 × 2.5 cm (1 ml); 1.6 × 2.5 cm (5 ml)
 
Recommended flow rate†  0.2-1 ml/min (1 ml); 0.5-5 ml/min (5 ml)
 
Max. pressure†  3 bar (0.3 MPa) 
* Binding of GST to glutathione is flow-rate dependent and lower flow rates often iincrease the binding capacity. This is an important consideration, especially during sample loading and elution.
† H2O at 25°C.

 

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